anti–cd27-pecy7 (Thermo Fisher)
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Anti–Cd27 Pecy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd27+pecy7/pm36858158-37-48-51
Average 90 stars, based on 1 article reviews
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Incubation:Article Title: Anti-idiotypic antibodies elicit anti-HIV-1–specific B cell responses Article Snippet: Enriched B cells were resuspended in 200 μl EasySep buffer and incubated with 10 μl rat serum, 10 μl mouse serum, 10 μl mouse–anti-human CD32 (551900; BD), 10 μl mouse–anti-human CD23 (555707; BD), and 10 μl mouse–anti-human CD16 (550383; BD). .. Cells were then washed with EasySep buffer and resuspended in 200 μl EasySep buffer and incubated with 5 μl of Zenon APC-DL755 and 5 μl of Zenon PE-DL650 and incubated on ice for 10 min. Next, B cells were stained with iv8 conjugated to either Zenon-PE or Zenon-APC and CD19-BV711 (302246; BioLegend) at 1:200 dilution, Staining:Article Title: Anti-idiotypic antibodies elicit anti-HIV-1–specific B cell responses Article Snippet: Enriched B cells were resuspended in 200 μl EasySep buffer and incubated with 10 μl rat serum, 10 μl mouse serum, 10 μl mouse–anti-human CD32 (551900; BD), 10 μl mouse–anti-human CD23 (555707; BD), and 10 μl mouse–anti-human CD16 (550383; BD). .. Cells were then washed with EasySep buffer and resuspended in 200 μl EasySep buffer and incubated with 5 μl of Zenon APC-DL755 and 5 μl of Zenon PE-DL650 and incubated on ice for 10 min. Next, B cells were stained with iv8 conjugated to either Zenon-PE or Zenon-APC and CD19-BV711 (302246; BioLegend) at 1:200 dilution, |



![Phenotyping residual memory and plasma cells after RTX (A) . Median absolute counts of unswitched memory <t>(CD27</t> + IgM + and/or IgD + ), switched memory (CD27 + IgG + or IgA + ) and double negative (DN, CD27 − , IgG + , or IgA + ) B-cells are shown for AAV patients (n = 6) after remission-induction treatment with RTX (B) . Median ± IQR fold change from baseline for different memory B-cell subsets are shown for each timepoint after RTX (C) . Median absolute counts of PBs (CD20 + CD138 − , red), PrePCs (CD20 − CD138 − , purple) and mPCs (CD20 − CD138 + , blue) are shown for AAV patients (n = 6) after RTX as remission-induction therapy (D) . Median [IQR] fold change from baseline for the subsets in A are shown for each timepoint after RTX. Red dashed line indicated the detection limit for HSFC. Gray area indicates 1–20 analyzed events.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0159/pmc07770159/pmc07770159__fimmu-11-566732-g003.jpg)