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anti–cd27-pecy7  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti–cd27-pecy7
    Anti–Cd27 Pecy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd27+pecy7/pm36858158-37-48-51
    Average 90 stars, based on 1 article reviews
    anti–cd27-pecy7 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Anti-idiotypic antibodies elicit anti-HIV-1–specific B cell responses
    Article Snippet: Enriched B cells were resuspended in 200 μl EasySep buffer and incubated with 10 μl rat serum, 10 μl mouse serum, 10 μl mouse–anti-human CD32 (551900; BD), 10 μl mouse–anti-human CD23 (555707; BD), and 10 μl mouse–anti-human CD16 (550383; BD). .. Cells were then washed with EasySep buffer and resuspended in 200 μl EasySep buffer and incubated with 5 μl of Zenon APC-DL755 and 5 μl of Zenon PE-DL650 and incubated on ice for 10 min. Next, B cells were stained with iv8 conjugated to either Zenon-PE or Zenon-APC and CD19-BV711 (302246; BioLegend) at 1:200 dilution, CD27-PECy7 (25-0271-82; eBioscience ) at a 1:600 dilution, CD14- FITC (557153; BD) at 1:60 dilution, CD3-FITC (556611; BD) at a 1:60 dilution, CD20-AF700 (302322; BioLegend) at a 1:300 dilution, IgD-PerCP-Cy5.5 (46-9868-42; eBioscience) at a 1:120 dilution, IgM-BV605 (314524; BioLegend) at a 1:120 dilution, and Fixable Viability Dye eFluor 506 (65-0866-14; eBioscience) at a 1:300 dilution. ..

    Staining:

    Article Title: Anti-idiotypic antibodies elicit anti-HIV-1–specific B cell responses
    Article Snippet: Enriched B cells were resuspended in 200 μl EasySep buffer and incubated with 10 μl rat serum, 10 μl mouse serum, 10 μl mouse–anti-human CD32 (551900; BD), 10 μl mouse–anti-human CD23 (555707; BD), and 10 μl mouse–anti-human CD16 (550383; BD). .. Cells were then washed with EasySep buffer and resuspended in 200 μl EasySep buffer and incubated with 5 μl of Zenon APC-DL755 and 5 μl of Zenon PE-DL650 and incubated on ice for 10 min. Next, B cells were stained with iv8 conjugated to either Zenon-PE or Zenon-APC and CD19-BV711 (302246; BioLegend) at 1:200 dilution, CD27-PECy7 (25-0271-82; eBioscience ) at a 1:600 dilution, CD14- FITC (557153; BD) at 1:60 dilution, CD3-FITC (556611; BD) at a 1:60 dilution, CD20-AF700 (302322; BioLegend) at a 1:300 dilution, IgD-PerCP-Cy5.5 (46-9868-42; eBioscience) at a 1:120 dilution, IgM-BV605 (314524; BioLegend) at a 1:120 dilution, and Fixable Viability Dye eFluor 506 (65-0866-14; eBioscience) at a 1:300 dilution. ..



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    Phenotyping residual memory and plasma cells after RTX (A) . Median absolute counts of unswitched memory <t>(CD27</t> + IgM + and/or IgD + ), switched memory (CD27 + IgG + or IgA + ) and double negative (DN, CD27 − , IgG + , or IgA + ) B-cells are shown for AAV patients (n = 6) after remission-induction treatment with RTX (B) . Median ± IQR fold change from baseline for different memory B-cell subsets are shown for each timepoint after RTX (C) . Median absolute counts of PBs (CD20 + CD138 − , red), PrePCs (CD20 − CD138 − , purple) and mPCs (CD20 − CD138 + , blue) are shown for AAV patients (n = 6) after RTX as remission-induction therapy (D) . Median [IQR] fold change from baseline for the subsets in A are shown for each timepoint after RTX. Red dashed line indicated the detection limit for HSFC. Gray area indicates 1–20 analyzed events.
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    Image Search Results


    List of antibodies used for immunophenotyping.

    Journal: Frontiers in Immunology

    Article Title: The impact on colostrum oxidative stress, cytokines, and immune cells composition after SARS-CoV-2 infection during pregnancy

    doi: 10.3389/fimmu.2022.1031248

    Figure Lengend Snippet: List of antibodies used for immunophenotyping.

    Article Snippet: Anti-CD27-PECy7 , Mouse IgG1, κ , M-T271 , 560609, BD Biosciences.

    Techniques:

    Cell populations in the colostrum of symptomatic (n=14) and asymptomatic (n=12) for Covid-19 of lactating women, during pregnancy. (A) IgA colostrum concentration (ng mL -1 ). (B) Total cell count (x10 7 mL -1 ). (C) Percentage of cells CD3-CD19 - CD138 + CD27 ++ . (D) Percentage of cells CD3 - CD19 + CD138 - CD27 - . (E) Percentage of cells CD3 - CD19 + CD138 - CD27 + . (F) Percentage of cells CD3 + CD4 + CD45RO - CD62L + . (G) Percentage of cells CD3 + CD8 + CD45RO - CD62L + . (H) Percentage of cells CD3 + CD4 + CD45RO + CD62L + . (I) Percentage of cells D3 + CD8 + CD45RO + CD62L + . (J) Percentage of cells CD3 + CD4 + CD45RO + CD62L - . (L) Percentage of cells CD3 + CD8 + CD45RO + CD62L - . (K) Percentage of cells CD3 + CD4 + CD45RO - CD62L - . (M) Percentage of cells CD3 + CD8 + CD45RO - CD62L - . (N) Percentage of cells CD3 + CD4 + Tbet + . (O) Percentage of cells CD3 + CD4 + GATA3 + . (P) Percentage of cells CD3 + CD4 + RORγt + IL17A + . (Q) Percentage of cells CD3 - CD4 - Tbet + . (R) Percentage of cells CD3 - CD4 - RORγt + . (S) Percentage of cells CD3 - CD4 - GATA3 + . (T) Percentage of cells CD3 - CD56 bright CD16 - CD27 + IFN-γ + . (U) Percentage of cells CD3 - CD56 dim CD16 + CD27 - . Data are presented as median and 95% CI. Mann–Whitney test: p < 0.05. * p < 0.000. ** p = 0.0093.

    Journal: Frontiers in Immunology

    Article Title: The impact on colostrum oxidative stress, cytokines, and immune cells composition after SARS-CoV-2 infection during pregnancy

    doi: 10.3389/fimmu.2022.1031248

    Figure Lengend Snippet: Cell populations in the colostrum of symptomatic (n=14) and asymptomatic (n=12) for Covid-19 of lactating women, during pregnancy. (A) IgA colostrum concentration (ng mL -1 ). (B) Total cell count (x10 7 mL -1 ). (C) Percentage of cells CD3-CD19 - CD138 + CD27 ++ . (D) Percentage of cells CD3 - CD19 + CD138 - CD27 - . (E) Percentage of cells CD3 - CD19 + CD138 - CD27 + . (F) Percentage of cells CD3 + CD4 + CD45RO - CD62L + . (G) Percentage of cells CD3 + CD8 + CD45RO - CD62L + . (H) Percentage of cells CD3 + CD4 + CD45RO + CD62L + . (I) Percentage of cells D3 + CD8 + CD45RO + CD62L + . (J) Percentage of cells CD3 + CD4 + CD45RO + CD62L - . (L) Percentage of cells CD3 + CD8 + CD45RO + CD62L - . (K) Percentage of cells CD3 + CD4 + CD45RO - CD62L - . (M) Percentage of cells CD3 + CD8 + CD45RO - CD62L - . (N) Percentage of cells CD3 + CD4 + Tbet + . (O) Percentage of cells CD3 + CD4 + GATA3 + . (P) Percentage of cells CD3 + CD4 + RORγt + IL17A + . (Q) Percentage of cells CD3 - CD4 - Tbet + . (R) Percentage of cells CD3 - CD4 - RORγt + . (S) Percentage of cells CD3 - CD4 - GATA3 + . (T) Percentage of cells CD3 - CD56 bright CD16 - CD27 + IFN-γ + . (U) Percentage of cells CD3 - CD56 dim CD16 + CD27 - . Data are presented as median and 95% CI. Mann–Whitney test: p < 0.05. * p < 0.000. ** p = 0.0093.

    Article Snippet: Anti-CD27-PECy7 , Mouse IgG1, κ , M-T271 , 560609, BD Biosciences.

    Techniques: Concentration Assay, Cell Counting, MANN-WHITNEY

    Gate strategies and representative dot plot graphs from flow cytometry data of two women (Asymptomatic number 7 and Symptomatic number 4). (A) CD3 - CD19 - and CD3 - CD19 + cells were gated for CD138 and CD27 analyzis. (B) CD3 + cells were further evaluated for CD4 or CD8. CD3 + CD4 + and CD3 + CD8 + cells were gated for CD45RO and CD62L analyzis. (C) CD3 - cells were evaluated for CD56. Both CD56 bright and CD56 dim were further analyzed for CD16 and CD27 expression. CD3 - CD56 bright CD16 - CD27 + cells were evaluated for IFN-γ analyzis. (D) CD3 + CD4 + and CD3 - CD4 - were evaluated for tbet, GATA3, and RORγt. CD3 + CD4 + RORγt + were also analyzed for IL17A.

    Journal: Frontiers in Immunology

    Article Title: The impact on colostrum oxidative stress, cytokines, and immune cells composition after SARS-CoV-2 infection during pregnancy

    doi: 10.3389/fimmu.2022.1031248

    Figure Lengend Snippet: Gate strategies and representative dot plot graphs from flow cytometry data of two women (Asymptomatic number 7 and Symptomatic number 4). (A) CD3 - CD19 - and CD3 - CD19 + cells were gated for CD138 and CD27 analyzis. (B) CD3 + cells were further evaluated for CD4 or CD8. CD3 + CD4 + and CD3 + CD8 + cells were gated for CD45RO and CD62L analyzis. (C) CD3 - cells were evaluated for CD56. Both CD56 bright and CD56 dim were further analyzed for CD16 and CD27 expression. CD3 - CD56 bright CD16 - CD27 + cells were evaluated for IFN-γ analyzis. (D) CD3 + CD4 + and CD3 - CD4 - were evaluated for tbet, GATA3, and RORγt. CD3 + CD4 + RORγt + were also analyzed for IL17A.

    Article Snippet: Anti-CD27-PECy7 , Mouse IgG1, κ , M-T271 , 560609, BD Biosciences.

    Techniques: Flow Cytometry, Expressing

    Compared to NKG2D(z) CAR-T cells, NKG2D(bbz) CAR-T cells exhibited reduced differentiation and exhaustion, and the Tnaive cell subset was increased. (A) CD27, CD28, CD127, CD25, CD62L, and PD1 on the surface of NKG2D(z) and NKG2D(bbz) CAR-T cells were detected by flow cytometry at 9 days after initial activation. The curves represent the flow cytometry peak map, and the bars represent the statistical map of the corresponding marker. (B) T cell subsets of NKG2D(z) and NKG2D(bbz) CAR-T cells were analyzed. T cell subsets are divided into naïve (CD45RA+, CCR7+), effector memory (em) (CD45RA−, CCR7−), central memory (cm) (CD45RA−, CCR7+), terminal effectors re-expressing CD45RA (emRA) (CD45RA+, CCR7−), and stem cell-like memory (scm) (CD45RA+, CD45RO+). Data come from ≥3 donors. p <0.05 indicates significant difference. Data are presented as the mean ± SD.

    Journal: Frontiers in Oncology

    Article Title: Combination of 4-1BB and DAP10 promotes proliferation and persistence of NKG2D(bbz) CAR-T cells

    doi: 10.3389/fonc.2022.893124

    Figure Lengend Snippet: Compared to NKG2D(z) CAR-T cells, NKG2D(bbz) CAR-T cells exhibited reduced differentiation and exhaustion, and the Tnaive cell subset was increased. (A) CD27, CD28, CD127, CD25, CD62L, and PD1 on the surface of NKG2D(z) and NKG2D(bbz) CAR-T cells were detected by flow cytometry at 9 days after initial activation. The curves represent the flow cytometry peak map, and the bars represent the statistical map of the corresponding marker. (B) T cell subsets of NKG2D(z) and NKG2D(bbz) CAR-T cells were analyzed. T cell subsets are divided into naïve (CD45RA+, CCR7+), effector memory (em) (CD45RA−, CCR7−), central memory (cm) (CD45RA−, CCR7+), terminal effectors re-expressing CD45RA (emRA) (CD45RA+, CCR7−), and stem cell-like memory (scm) (CD45RA+, CD45RO+). Data come from ≥3 donors. p <0.05 indicates significant difference. Data are presented as the mean ± SD.

    Article Snippet: CAR-T cell phenotypes were assessed with monoclonal antibodies against the following molecules: CD4 (FITC, Biolegend), CD8 (PE, Biolegend), PD1 (APC, Biolegend), Tim3 (FITC, Biolegend), LAG3 (PE, Biolegend), CD27 (PECY7, BD), CD28 (PECY7, BD), CD25 (APC, Biolegend), CD127 (APC, Biolegend), CD62L (PE/PB, Biolegend), CD45RA (APC/PE, Biolegend), CCR7 (FITC, Biolegend), annexin V (APC, Biolegend), and 7-aminoactinomycin D (7AAD; ThermoFisher).

    Techniques: Flow Cytometry, Activation Assay, Marker, Expressing

    Journal: eLife

    Article Title: The oxygen sensor prolyl hydroxylase domain 2 regulates the in vivo suppressive capacity of regulatory T cells

    doi: 10.7554/eLife.70555

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-mouse CD27-PeCy7 (LG.7F9, mouse monoclonal) , eBioscience , 25-0271-82 , (1:250).

    Techniques: Recombinant, Sequencing, Software

    Phenotyping residual memory and plasma cells after RTX (A) . Median absolute counts of unswitched memory (CD27 + IgM + and/or IgD + ), switched memory (CD27 + IgG + or IgA + ) and double negative (DN, CD27 − , IgG + , or IgA + ) B-cells are shown for AAV patients (n = 6) after remission-induction treatment with RTX (B) . Median ± IQR fold change from baseline for different memory B-cell subsets are shown for each timepoint after RTX (C) . Median absolute counts of PBs (CD20 + CD138 − , red), PrePCs (CD20 − CD138 − , purple) and mPCs (CD20 − CD138 + , blue) are shown for AAV patients (n = 6) after RTX as remission-induction therapy (D) . Median [IQR] fold change from baseline for the subsets in A are shown for each timepoint after RTX. Red dashed line indicated the detection limit for HSFC. Gray area indicates 1–20 analyzed events.

    Journal: Frontiers in Immunology

    Article Title: Highly Sensitive Flow Cytometric Detection of Residual B-Cells After Rituximab in Anti-Neutrophil Cytoplasmic Antibodies-Associated Vasculitis Patients

    doi: 10.3389/fimmu.2020.566732

    Figure Lengend Snippet: Phenotyping residual memory and plasma cells after RTX (A) . Median absolute counts of unswitched memory (CD27 + IgM + and/or IgD + ), switched memory (CD27 + IgG + or IgA + ) and double negative (DN, CD27 − , IgG + , or IgA + ) B-cells are shown for AAV patients (n = 6) after remission-induction treatment with RTX (B) . Median ± IQR fold change from baseline for different memory B-cell subsets are shown for each timepoint after RTX (C) . Median absolute counts of PBs (CD20 + CD138 − , red), PrePCs (CD20 − CD138 − , purple) and mPCs (CD20 − CD138 + , blue) are shown for AAV patients (n = 6) after RTX as remission-induction therapy (D) . Median [IQR] fold change from baseline for the subsets in A are shown for each timepoint after RTX. Red dashed line indicated the detection limit for HSFC. Gray area indicates 1–20 analyzed events.

    Article Snippet: Cells were stained in the dark for 30 min at 4°C with the following antibody panel: anti-CD45-PerCP, anti-CD3-FITC, anti-CD14-APC-H7 (BD Biosciences, Franklin Lakes, NJ, USA), anti-IgD-PE, anti-CD19-eFluor450, anti-CD27-PeCy7 (Thermo Fisher Scientific, Waltham, MA, USA), and anti-CD38-APC (BioLegend, San Diego, CA, USA).

    Techniques:

    Minimal residual autoimmunity after RTX: presence of ANCA-specific memory B-cells. 1*10 6 PBMCs/well from healthy controls (HCs) and AAV patients before, 24 and 48 weeks after RTX treatment, were stimulated for 10 days with CpG ODN class B, IL-2, and IL-21 to induce antibody-secreting cells (ASCs) in a 48-well plate (A) . Representative bivariate dot plots of ASCs at day 0 and day 7 of PBMC cultures demonstrated the induction of CD27 ++ CD38 ++ ASCs 7 days after polyclonal stimulation of PBMCs from a HC and an AAV patient (MPO-ANCA) (B) . Absolute counts of total CD19 + B-cells were shown for each individual at baseline of the cultures (day 0) (C) . Absolute counts of induced ASCs per well were shown for each individual after 7 days of culturing (D) . Total IgG production was measured in the supernatants of each well after 10 days of culturing. Here the median of 5 wells is shown per individual (E) . Total ANCA-IgG production was measured in the supernatants of each well after 10 days of culturing. Anti-PR3 IgG and anti-MPO IgG are, respectively, shown on the left and right y-axis. Each dot represents the median of 5 wells for each individual sample. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Highly Sensitive Flow Cytometric Detection of Residual B-Cells After Rituximab in Anti-Neutrophil Cytoplasmic Antibodies-Associated Vasculitis Patients

    doi: 10.3389/fimmu.2020.566732

    Figure Lengend Snippet: Minimal residual autoimmunity after RTX: presence of ANCA-specific memory B-cells. 1*10 6 PBMCs/well from healthy controls (HCs) and AAV patients before, 24 and 48 weeks after RTX treatment, were stimulated for 10 days with CpG ODN class B, IL-2, and IL-21 to induce antibody-secreting cells (ASCs) in a 48-well plate (A) . Representative bivariate dot plots of ASCs at day 0 and day 7 of PBMC cultures demonstrated the induction of CD27 ++ CD38 ++ ASCs 7 days after polyclonal stimulation of PBMCs from a HC and an AAV patient (MPO-ANCA) (B) . Absolute counts of total CD19 + B-cells were shown for each individual at baseline of the cultures (day 0) (C) . Absolute counts of induced ASCs per well were shown for each individual after 7 days of culturing (D) . Total IgG production was measured in the supernatants of each well after 10 days of culturing. Here the median of 5 wells is shown per individual (E) . Total ANCA-IgG production was measured in the supernatants of each well after 10 days of culturing. Anti-PR3 IgG and anti-MPO IgG are, respectively, shown on the left and right y-axis. Each dot represents the median of 5 wells for each individual sample. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Cells were stained in the dark for 30 min at 4°C with the following antibody panel: anti-CD45-PerCP, anti-CD3-FITC, anti-CD14-APC-H7 (BD Biosciences, Franklin Lakes, NJ, USA), anti-IgD-PE, anti-CD19-eFluor450, anti-CD27-PeCy7 (Thermo Fisher Scientific, Waltham, MA, USA), and anti-CD38-APC (BioLegend, San Diego, CA, USA).

    Techniques: